ELISA Microplate Reader yog dab tsi?
Tus nyeem ntawv microplate, uas yog, enzyme-linked immunosorbent assay, yog ib qho cuab yeej tshwj xeeb rau enzyme-linked immunosorbent assay. Qhov tseeb, nws yog ib qho tshwj xeeb photoelectric colorimeter los yog spectrophotometer nyob rau hauv disguise, thiab nws cov hauv paus ntsiab lus ntawm kev ua hauj lwm yog ib yam nkaus li lub ntsiab qauv thiab photoelectric colorimeter. Nws tuaj yeem muab faib ua ob pawg, semi-automatic thiab tag nrho tsis siv neeg, tab sis lawv cov qauv kev ua haujlwm yog tib yam. Nws lub hauv paus yog colorimeter, uas yog, txoj kev colorimetric yog siv los soj ntsuam cov ntsiab lus ntawm antigen lossis antibody. Kev txiav txim siab ELISA feem ntau yuav tsum muaj qhov kawg ntim ntawm cov tshuaj ntsuam xyuas kom tsawg dua 250μL, thiab qhov kev xeem tsis tuaj yeem ua tiav nrog lub tshuab photoelectric colorimeter, yog li muaj cov kev cai tshwj xeeb rau photoelectric colorimeter hauv microplate nyeem ntawv.
Txoj Cai
Tus nyeem ntawv microplate yog qhov tshwj xeeb-lub hom phiaj photoelectric colorimeter lossis spectrophotometer hauv kev zais, thiab nws txoj hauv kev ua haujlwm tseem ceeb yog tib yam li cov qauv tseem ceeb thiab photoelectric colorimeter. Daim duab qhia txog kev ua haujlwm ntawm ib leeg-channel tsis siv neeg sampling microplate nyeem ntawv. Schematic daim duab. Lub teeb nthwv dej tawm los ntawm lub teeb lub teeb tau hloov mus rau hauv lub teeb ci monochromatic los ntawm lub lim lossis monochromator, thiab nkag mus rau cov qauv hauv qab kev sim hauv cov yas microporous ncej. Ib feem ntawm lub teeb monochromatic yog absorbed los ntawm cov qauv, thiab lwm qhov yog kis los ntawm cov qauv Illuminated mus rau lub photodetector, lub photodetector converts lub teeb sib txawv ntawm cov qauv coj mus kuaj rau cov khoom siv hluav taws xob. Cov teeb liab hluav taws xob tau ua tiav los ntawm kev ua ntej amplification, logarithmic amplification, thiab analog-to-digital conversion. Nkag mus rau microprocessor rau kev ua cov ntaub ntawv thiab kev suav, thiab thaum kawg tso tawm cov txiaj ntsig los ntawm cov zaub thiab lub tshuab luam ntawv. Lub microprocessor tseem tswj cov kev txav ntawm lub tshuab tsav tsheb hauv X thiab Y kev taw qhia kom txav lub microplate los ntawm kev tswj xyuas Circuit Court, kom paub txog cov txheej txheem kuaj tsis siv neeg. Lwm cov neeg nyeem ntawv microplate siv phau ntawv txav ntawm lub microplate rau kev kuaj pom, yog li lub tshuab tsav tsheb thiab tswj Circuit Court hauv X thiab Y cov lus qhia raug tshem tawm, kom lub ntsuas me me thiab cov qauv yooj yim dua.
Lub microplate yog pob tshab yas phaj tshwj xeeb yog siv los tso cov qauv mus kuaj. Lub phaj muaj ntau kab ntawm qhov me me ntawm qhov sib xws, thiab cov tshuaj tiv thaiv kab mob los yog cov tshuaj tiv thaiv kab mob yog embedded rau hauv lub qhov. Txhua qhov me me tuaj yeem tuav ob peb kaum milliliters ntawm kev daws.
Lub teeb yog electromagnetic yoj. Lub wavelength ntawm 100nm-400nm yog hu ua lub teeb ultraviolet, lub teeb ntawm 400nm thiab 780nm tuaj yeem pom los ntawm tib neeg lub qhov muag, thiab lub wavelength ntau dua 780nm hu ua lub teeb infrared. Tib neeg tsuas tuaj yeem pom cov xim vim tias lub teeb cuam tshuam cov khoom thiab rov qab los ntawm cov khoom. Cov nroj tsuag ntsuab yog ntsuab vim hais tias feem ntau cov nroj tsuag nqus liab-txiv kab ntxwv lub teeb thiab xiav-violet lub teeb, tab sis lawv tsis nqus thiab xav txog ntsuab, yog li cov nroj tsuag tshwm ntsuab. Lub hauv paus ntsiab lus ntawm kev ntsuas microplate nyeem ntawv yog txhawm rau txheeb xyuas qhov nqus ntawm qhov ntsuas ntawm qhov tshwj xeeb wavelength.
Qauv
Muaj 40-zoo daim hlau, 55-zoo daim hlau, 96-zoo daim hlau, thiab lwm yam. Cov cuab yeej sib txawv siv cov phaj sib txawv, uas tuaj yeem kuaj ib qho los ntawm ib qho los yog kab los ntawm kab.
Lub teeb monochromatic siv los ntawm tus nyeem ntawv microplate tuaj yeem tau txais los ntawm cov lim coherent lossis tib lub monochromator raws li spectrophotometer. Thaum siv lub lim raws li cov khoom siv lim, nws yog tib yam li cov xim zoo tib yam. Nws tuaj yeem muab tso rau pem hauv ntej ntawm lub microplate lossis tom qab lub microplate. Tom qab lub condenser, lub diaphragm ncav cuag lub reflector, nws yog reflected los ntawm lub reflector ntawm 90 ° thiab hla los ntawm cov colorimetric tov vertically, thiab ces xa los ntawm lub lim. Mus rau lub photocell.
Los ntawm daim duab thaiv kev ua haujlwm thiab daim duab qhia kev teeb pom kev ntawm tus nyeem ntawv microplate, nws tuaj yeem pom tau tias nws muaj qhov sib txawv hauv qab no nrog cov duab photoelectric colorimeters:
(L) Lub thawv rau cov tshuaj colorimetric yuav tsum tsis txhob siv ib tug cuvette, tab sis ib tug yas microplate. Lub microplate feem ntau yog ua los ntawm cov khoom siv pob tshab polyethylene, uas muaj cov nyhuv adsorption muaj zog ntawm antigen thiab antibody, yog li nws yog siv los ua cov khoom thauj theem.
⑵Vim tias cov yas microplate uas muaj cov qauv yog ntau kab ntxeem tau, lub teeb tsuas tuaj yeem dhau los ntawm vertically, yog li lub teeb beam ntawm tus nyeem ntawv microplate dhau los ntawm kev tshuaj ntsuam xyuas thiab lub microplate vertically. Lub teeb beam tuaj yeem yog los ntawm sab saum toj mus rau hauv qab lossis Nws tuaj yeem dhau los ntawm cov kua dej colorimetric los ntawm hauv qab mus rau sab saum toj.
⑶ Cov nyeem ntawv Microplate feem ntau tsis tsuas yog siv A, tab sis qee zaum kuj siv optical ceev OD los qhia txog kev nqus. Microplate nyeem ntawv tuaj yeem muab faib ua ob hom: ib leeg-channel thiab ntau-channel, thiab ib-channel muab faib ua ob hom: tsis siv neeg thiab phau ntawv. Tsis siv neeg hom twj muaj ib lub tshuab tsav tsheb hauv X thiab Y cov lus qhia, uas tuaj yeem xa cov qhov me me ntawm microplate L ib qho los ntawm ib qho hauv qab kab rau kev sim, thiab hom phau ntawv siv phau ntawv txav ntawm lub microplate rau kev ntsuas.
Nyob rau hauv lub hauv paus ntawm ib-channel microplate nyeem ntawv, ib tug multi-channel microplate nyeem ntawv tau tsim. Hom microplate nyeem ntawv no feem ntau yog automated. Nws tsis muaj ntau kab teeb thiab ntau lub photodetectors, xws li 12-channel ntsuas nrog 12 kab teeb lossis 12 lub teeb pom kev zoo, 12 detectors thiab 12 amplifiers, nyob rau hauv qhov kev txiav txim ntawm cov cuab yeej tsav tsheb hauv X kev taw qhia, 12 cov qauv kuaj pom hauv ib qho kab. Qhov kev tshawb nrhiav ceev ntawm cov nyeem ntawv multi-channel microplate yog ceev, tab sis nws cov qauv ntau cov nqi kuj tseem siab dua.
Daim ntawv thov ntau ntawm microplate nyeem ntawv
Hematology test Platelet-related antibody test PAIgA, PAIgG, PAIgM
Kev txiav txim siab ntawm D-Dimer D-Dimer
Kev txiav txim siab ntawm serum fibrin degradation khoom FDP
Kev txiav txim siab ntawm T3, T4 nrog triiodothyronine thiab tetraiodothyronine
Immunological test C-reactive protein txiav txim CRP
Kev txiav txim siab ntawm immunoglobulin IgD, IgE
Kev txiav txim siab ntawm circulating immune complexes CIC
Kev txiav txim siab ntawm rheumatoid factor IgG, IgA, IgM RF
Anti-thyroglobulin antibody, microsomal antibody determination TG, TM
qog nqaij hlav cancer immunological kev kuaj pom Alpha-fetoprotein kev txiav txim AFP
Kev txiav txim siab ntawm carcinoembryonic antigen CEA
Kev txiav txim siab ntawm prostate tshwj xeeb antigen PSA
Kev txiav txim siab ntawm mob qog noj ntshav pancreatic, mob qog noj ntshav biliary, mob plab hnyuv CA19-9
Kev txiav txim siab ntawm zes qe menyuam qog noj ntshav CA125
Kev txiav txim siab ntawm mob qog noj ntshav mis CA15-3
Kev txiav txim siab ntawm ncauj tsev menyuam squamous cell carcinoma SCC
Kev tshawb nrhiav ntau yam mob qog nqaij hlav hauv pob txha
Kev txiav txim siab ntawm thyroid cancer hTG
Infectious disease immunological test, hepatitis A serological test, anti-HAV-IgM
Hepatitis B Serology Test Ob thiab ib nrab khub
Hepatitis C serology detection Anti-HAV-IgG, anti-HCV-IgM
Kev kuaj xyuas kab mob siab D Anti-HDV-IgG, anti-HDV-IgM
Serological detection of hepatitis E Anti-HEV-IgG
Kev kuaj xyuas cov tshuaj tiv thaiv rau hemorrhagic fever nrog raum mob HFRS-IgG
Kev kuaj pom tus kab mob Japanese encephalitis antibody IgM
Kev kuaj pom ntawm tib neeg cov tshuaj tiv thaiv kab mob tiv thaiv kab mob (piv txwv li, regional AIDS) HIV β 2 M
Prenatal and postnatal care function test Toxoplasma virus test TOXO
Rubella Virus Detection RV
Cytomegalovirus detection CMV
Kev kuaj pom tus kab mob herpes simplex Anti-HSV (Ⅰ, Ⅱ)
Lwm tus tuaj yeem ua cov tshuaj ntsuam genetic tests thiab tsiaj seem, tshuaj tua kab residues tshuaj xyuas cov khoom, thiab lwm yam.
