Lub hauv paus ntsiab lus ntawm magnetic hlaws nucleic acid extractor yog dab tsi?
Nucleic acid extraction tshuabKev faib tawm Nucleic acid extraction instrument yog ib qho cuab yeej siv sib txuam nucleic acid extraction reagents kom ua tiav cov qauv nucleic acid extraction.
Nucleic acid extraction tshuab tuaj yeem muab faib ua ob pawg: ib qho yog loj -scale automation, feem ntau hu ua tsis siv neeg ua kua ua haujlwm; lwm qhov yog loj - nplai automated kua workstation; lwm yam yog me me nucleic acid tsis siv neeg extractor, uas siv cov ntim reagents kom tiav cov extraction thiab purification txheej txheem. Loj - nplai tsis siv neeg ua kua ua haujlwm tsis tshua muaj siv vim cov cuab yeej siv siab thiab cov nqi khiav lag luam, thiab tsim nyog rau kev rho tawm ntau txhiab tus qauv zoo sib xws hauv ib lub sijhawm; Cov twj siv me me tsis siv neeg tau siv dav vim lawv cov nqi qis thiab kev ua haujlwm yooj yim.
Daim ntawv thov ntawm nucleic acid extraction tshuab
Hauv yuav luag txhua lub chaw kuaj mob, kev sib cais thiab kev ua kom huv ntawm biomolecules yog qhov tseem ceeb thiab tsim nyog. Txawm li cas los xij, purifying ntau cov qauv yog qhov nyuaj heev. Nws tsis yog tsuas yog tsim nyog los xaiv cov txheej txheem purification uas tsim nyog, tab sis kuj tseem muaj qhov ua haujlwm loj tshwj xeeb, uas nyuaj rau kev ua tau raws li qhov xav tau ntawm kev loj hlob sai ntawm siab -throughput qauv extraction thiab purification.
Nucleic acid extractor Lub nucleic acid extractor yog ib qho cuab yeej siv uas txhawb nqa nucleic acid extraction reagents kom tau txais nucleic acid extraction los ntawm cov qauv. Nws tau siv dav hauv kev kuaj kab mob microbial ib puag ncig, kuaj kev nyab xeeb khoom noj, kev nyab xeeb ntawm kev xa ntshav, kev txheeb xyuas qhov tseeb, chaw tswj kab mob, kuaj kab mob, kev tshawb fawb lom neeg thiab tsiaj txhu, thiab lwm yam.
1. Kev sib cais ntawm nucleic acid los ntawm kev sib nqus pas nrig yog pom tau los ntawm kev hloov ntawm cov hlaws sib nqus thiab cov immobilization ntawm cov kua. Lub hauv paus ntsiab lus thiab txheej txheem yog tib yam li txoj kev nqus. Cov hlaws sib nqus tau sib cais los ntawm cov kua sib txawv. Txoj kev sib nqus pas nrig siv cov pas nrig sib nqus los nqus cov hlaws sib nqus, cais cov hlaws sib nqus los ntawm cov kua khib nyiab, thiab muab tso rau hauv cov kua khib nyiab hauv kauj ruam tom ntej kom ua tiav nucleic acid extraction.
2. Suction method Suction method, tseem hu ua pipetting method, siv kua hloov los yog sib nqus hlaws fixation kom tiav nucleic acid extraction. Feem ntau, kev hloov pauv tau ua tiav los ntawm kev ua haujlwm ntawm lub kaw lus tswj kev tswj hwm, suav nrog cov txheej txheem rho tawm hauv qab no:
1. Pyrolysis: Ntxiv cov tshuaj pyrolysis rau cov qauv, sib tov cov tshuaj tiv thaiv, thiab ua kom tiav cov tshuaj tiv thaiv los ntawm kev siv tshuab thiab cua sov. Txiav cov hlwb, tso cov nucleic acids
2. Adsorption: Ntxiv cov hlaws sib nqus rau hauv cov qauv lysate, sib tov kom zoo, thiab siv cov hlaws sib nqus los adsorb nucleic acids vim tias lawv muaj kev sib raug zoo rau nucleic acids nyob rau hauv siab ntsev thiab tsis tshua muaj pH. Nyob rau hauv qhov kev txiav txim ntawm sab nraud magnetic teb, cov hlaws sib nqus tau sib cais los ntawm kev daws, thiab cov hlaws sib nqus tau xa mus rau cov kua dej khib nyiab los ntawm qhov nqus cov ntsiab lus, thiab cov lus nqus nqus tau muab pov tseg.
3. Kev ntxuav: tshem tawm cov hlau nplaum sab nraud, ntxiv cov tshuaj ntxuav ntawm lub hau tshiab, do sib npaug, tshem tawm cov impurities, thiab tshem tawm cov kua hauv qab qhov kev txiav txim ntawm sab nraud magnetic teb.
4. Elution: tshem tawm sab nraud magnetic teb, ntxiv elution buffer nrog ib tug tshiab ntsis, sib tov zoo, thiab cais cov bound nucleic acid los ntawm cov magnetic hlaws dai kom tau purified nucleic acid.
(1) Lub nucleic acid extractor yuav tsum yog tsawg kawg yog 10 cm deb ntawm lwm qhov chaw ntsug
(2) Lub zog hluav taws xob ntawm lub ntsuas hluav taws xob yuav tsum tau ua kom tsis txhob muaj hluav taws xob poob
(3) Tus neeg teb xov tooj yuav tsum tsis txhob disassemble lub twj paj nruag yam tsis tau kev tso cai. Kev hloov cov khoom siv lossis kev hloov kho sab hauv yuav tsum tau ua los ntawm cov kws tshaj lij kev pabcuam uas muaj ntawv tso cai. Tsis txhob hloov qhov chaw thaum lub hwj chim qhib
(4) Cov av noo ntawm cov txheeb ze yog 10 feem pua -80 feem pua, cov cua ntws zoo ntawm 35 degree lossis 40 degree qis dua qhov qub atmospheric siab; Qhov siab tshaj plaws yuav tsum qis dua 3000 meters, qhov kub yog 20-35 degree, thiab kev ua haujlwm kub yog 25 degree.
(5) Tsis txhob tso lub ntsuas hluav taws xob ze ntawm qhov chaw cua sov, xws li hluav taws xob cua sov. Tiv thaiv kom tsis txhob muaj dej los yog lwm yam kua los ntawm kev sib tsoo rau hauv cov khoom siv hluav taws xob kom tsis txhob muaj luv luv
(6) Cov cua nkag thiab qhov chaw tso tawm yog nyob rau sab nraum qab ntawm lub ntsuas. Nyob rau tib lub sijhawm, cov plua plav lossis cov fibers yuav tsum tiv thaiv kom tsis txhob sib xyaw hauv huab cua kom tsawg, thiab cov kav dej yuav tsum tau ceev kom meej.
