Dab tsi yog microplate nyeem ntawv?
Ntawd yog, qhov enzyme-linked immunosorbent assay yog ib qho cuab yeej tshwj xeeb rau enzyme-linked immunosorbent assay, tseem hu ua microplate detector. Nws tuaj yeem muab faib ua ob pawg: semi-automatic thiab tag nrho tsis siv neeg, tab sis lawv cov qauv kev ua haujlwm yog tib yam. Lub hauv paus yog ib tug colorimeter, uas yog, colorimetric tsom xam yog siv. Kev txiav txim siab feem ntau yuav tsum muaj qhov kawg ntim ntawm cov tshuaj ntsuam xyuas kom tsawg dua 250μL, thiab qhov kev xeem tsis tuaj yeem ua tiav nrog lub tshuab photoelectric colorimeter, yog li muaj cov kev cai tshwj xeeb rau photoelectric colorimeter hauv microplate nyeem ntawv.
txoj cai
Tus nyeem ntawv microplate yog qhov hloov pauv photoelectric colorimeter lossis spectrophotometer, thiab nws txoj hauv kev ua haujlwm tseem ceeb yog tib yam li cov qauv tseem ceeb thiab photoelectric colorimeter. Daim duab qhia tau hais tias cov qauv kev ua haujlwm ntawm ib leeg-channel tsis siv neeg sampling microplate nyeem ntawv Lub teeb nthwv dej tawm los ntawm lub teeb lub teeb tau hloov mus rau hauv lub teeb ci monochromatic los ntawm lim lossis monochromator, thiab nkag mus rau cov qauv hauv qab kev sim hauv cov yas microporous ncej. Ib feem ntawm lub teeb monochromatic yog absorbed los ntawm cov qauv, thiab lwm qhov yog irradiated los ntawm cov qauv. Ntawm lub photodetector, lub photodetector hloov cov teeb liab sib txawv ntawm cov qauv coj mus kuaj rau hauv cov khoom siv hluav taws xob sib xws. Cov teeb liab hluav taws xob tau ua tiav los ntawm kev ua ntej amplification, logarithmic amplification, thiab analog-to-digital conversion, thiab tom qab ntawd xa mus rau micro-processing Lub processor ua cov ntaub ntawv ua thiab suav, thiab thaum kawg qhia cov txiaj ntsig los ntawm cov zaub thiab lub tshuab luam ntawv. Lub microprocessor tseem tswj cov kev txav ntawm lub tshuab tsav tsheb hauv X thiab Y kev taw qhia kom txav lub microplate los ntawm kev tswj xyuas Circuit Court, kom paub txog cov txheej txheem kuaj tsis siv neeg. Tus nyeem ntawv microplate yog siv los txav lub microplate rau kev kuaj pom, yog li cov tshuab tsav tsheb thiab tswj Circuit Court hauv X thiab Y cov lus qhia raug tshem tawm, kom lub ntsuas me me thiab cov qauv yooj yim dua.
Tus nyeem ntawv microplate yog qhov hloov pauv photoelectric colorimeter lossis spectrophotometer, thiab nws txoj hauv kev ua haujlwm tseem ceeb yog tib yam li cov qauv tseem ceeb thiab photoelectric colorimeter. Daim duab qhia tau hais tias cov qauv kev ua haujlwm ntawm ib leeg-channel tsis siv neeg sampling microplate nyeem ntawv Lub teeb nthwv dej tawm los ntawm lub teeb lub teeb tau hloov mus rau hauv lub teeb ci monochromatic los ntawm lim lossis monochromator, thiab nkag mus rau cov qauv hauv qab kev sim hauv cov yas microporous ncej. Ib feem ntawm lub teeb monochromatic yog absorbed los ntawm cov qauv, thiab lwm qhov yog irradiated los ntawm cov qauv. Ntawm lub photodetector, lub photodetector hloov cov teeb liab sib txawv ntawm cov qauv coj mus kuaj rau hauv cov khoom siv hluav taws xob sib xws. Cov teeb liab hluav taws xob tau ua tiav los ntawm kev ua ntej amplification, logarithmic amplification, thiab analog-to-digital conversion, thiab tom qab ntawd xa mus rau micro-processing Lub processor ua cov ntaub ntawv ua thiab suav, thiab thaum kawg qhia cov txiaj ntsig los ntawm cov zaub thiab lub tshuab luam ntawv. Lub microprocessor tseem tswj cov kev txav ntawm lub tshuab tsav tsheb hauv X thiab Y kev taw qhia kom txav lub microplate los ntawm kev tswj xyuas Circuit Court, kom paub txog cov txheej txheem kuaj tsis siv neeg. Tus nyeem ntawv microplate yog siv los txav lub microplate rau kev kuaj pom, yog li cov tshuab tsav tsheb thiab tswj Circuit Court hauv X thiab Y cov lus qhia raug tshem tawm, kom lub ntsuas me me thiab cov qauv yooj yim dua.
siv
Nws tuaj yeem siv dav hauv thaj chaw qis-ultraviolet DNA, RNA kom muaj nuj nqis thiab purity tsom xam (A260/A280) thiab protein ntau (A280/BCA/Braford/Lowry), enzyme kev ua, enzyme kinetic nrhiav, enzyme-linked immunoassays (ELISAs), Cell Proliferation thiab toxicity analysis, cell apoptosis detection (MTT), reporter gene detection and G protein coupled receptor analysis (GPCR), etc.
